Transcriptional augmentation of hyaluronan production by Maitake-ethanol extract in human dermal fibroblasts

Purpose

Investigation of the effect of maitake mushroom extract on hyaluronic acid production in human skin fibroblasts.

Results

• fibroblasts, Maitake mushroom extract promoted HAS-2 mRNA expression in a concentration-dependent manner
• The amount of hyaluronic acid in the culture medium increased by maitake mushroom extract treatment due to the promotion of HAS-2 gene expression.
• This increase in hyaluronic acid was suppressed in a concentration-dependent manner by the hyaluronic acid synthase inhibitor 4-methylumbelliferone.
• Maitake mushroom extract was found to have no effect on type I collagen a1 and elastin gene expression.
• Maitake mushroom extract promoted HAS-2 and HAS-3 mRNA expression in human epidermal cells.

Discussion

• Maitake mushroom extract was found to promote hyaluronic acid production due to increased HAS-2 gene expression in human skin fibroblasts.
• It was suggested that maitake mushroom extract may be a specific promoter of hyaluronic acid in the regulation of extracellular matrix synthesis in the skin.